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Detection of pathogenic Yersinia enterocolitica by a rapid and sensitive duplex PCR assay

W J Wannet1, M Reessink, H A Brunings

  • 1National Institute of Public Health and the Environment, Bilthoven, The Netherlands. wim.wannet@rivm.nl

Journal of Clinical Microbiology
|November 29, 2001
PubMed

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...