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Molecular characterization of first human Bartonella strain isolated in Italy
A Ciervo1, A Petrucca, S Ciarrocchi
1Department of Bacteriology and Medical Mycology, Istituto Superiore di Sanità, 00161 Rome, Italy.
Abstract:
The aim of this study was to characterize a Bartonella strain (BA-1) isolated from a blood culture of an Italian, human immunodeficiency virus-positive patient with bacillary angiomatosis. We analyzed the isolate using molecular biology methods such as whole-cell fatty acid analysis, PCR-restriction fragment length polymorphism analysis, type-specific 16S rRNA PCRs, sequence analysis of the 16S rRNA, pulsed-field gel electrophoresis, and arbitrarily primed PCR. The BA-1 isolate turned out to be a Bartonella quintana strain, similar but not identical to B. quintana Oklahoma, which was used as a control strain.