Role of Intracellular Sodium Activity in the Control of Contraction in Cardiac Purkinje Fibers

The role of intracellular sodium activity (a(i)(Na)) in the control of force was studied in sheep cardiac Purkinje fibers exposed to norepinephrine (NE) and high [Ca](o) in the absence and presence of overdrive or of a low concentration of strophanthidin. Both NE and high [Ca](o) decrease a(i)(Na) and increae force, while overdrive increases and low strophanthidin decreases both parameters. In the presence of NE, overdrive increases a(i)(Na) less than force and is followed by a more pronounced undershoot in a(i)(Na) and force. In contrast, in high [Ca](o) overdrive increases a(i)(Na) more than force and is followed by a less pronounced undershoot in a(i)(Na) and force than in NE. High [Ca](o) increases force to a peak, but then the decreasing a(i)(Na) reduces force. In all these conditions, a(i)(Na) determines force changes during recovery from overdrive. NE and high [Ca](o) decrease a(i)(Na) less and increase force more in low strophanthidin. Thus, changes in a(i)(Na) modulate the increase in force due to increased Ca influx and control force development when Ca influx is either unchanged (low strophanthidin) or has reached a steady state (high [Ca](o), recovery from overdrive). Copyright 1994 S. Karger AG, Basel