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Published on: April 11, 2016
Using 2', 7'-dichlorodihydrofluorescein-diacetate to assess polysaccharides as immunomodulating agents
E J Bland1, T Keshavarz, C Bucke
1Fungal Biotechnology Group, School of Biosciences, University of Westminster, 115 New Cavendish Street, London, W1W 6UW, UK.
Abstract:
Neutrophils play a key role in the defense against microbial infections. One of their primary weapons to destroy microbes is the production of reactive oxygen species (ROS). This paper shows how 2', 7'-dichlorodihydrofluorescein-diacetate (DCFH-DA) was used to measure the effects of polysaccharides on the production of ROS by polymorphonuclear leukocytes (PMNs). The DCFH-DA method has been designed to provide a highly sensitive, quantifiable, real-time assessment of ROS production by PMNs.
