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An Efficient Method for the Isolation of Highly Purified RNA from Seeds for Use in Quantitative Transcriptome Analysis
Published on: January 11, 2017
Isolation of high quality RNA from bilberry (Vaccinium myrtillus L.) fruit
L Jaakola1, A M Pirttilä, M Halonen
1Department of Biology, University of Oulu, P.O. Box 3000, FIN-90014 Oulu, Finland. Laura.Jaakola@oulu.fi
Abstract:
A simple and efficient method is described for isolating high quality RNA from bilberry fruit. The procedure is based on the use of hexadecyltrimethyl ammonium bromide (CTAB), polyvinylpyrrolidone (PVP), and beta-mercaptoethanol in an extraction buffer in order to eliminate the polysaccharides and prevent the oxidation of phenolic compounds. This method is a modification of the one described for pine trees, and yields high-quality RNA suitable for cDNA based methodologies. This method is applicable for a variety of plant tissues.
