Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Sentinel lymph node detection and microstaging in vulvar carcinoma.

K L Molpus1, M C Kelley, J E Johnson

  • 1Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE 68198-3255, USA. kmolpus@unmc.edu

The Journal of Reproductive Medicine
|December 1, 2001
PubMed
Summary

Combined techniques improve sentinel lymph node (SLN) detection in vulvar cancer. Histologic microstaging further enhances the identification of micrometastases in SLNs, crucial for accurate cancer staging.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Diabetes Cured by Skim-milk.

The Chicago medical journal and examiner·2023
Same author

Public Hygiene and Legal Medicine: November, 1860.

Chicago medical examiner·2023
Same author

Observations on the Therapeutic Action of the Oxalate of Cerium in the Vomiting of Pregnancy.

The Chicago medical journal·2023
Same author

Retroversion of the Uterus, with a Description of an Instrument Designed for Its Reposition.

The Chicago medical journal·2023
Same author

Applications of automation in cervical cancer screening.

Journal of lower genital tract disease·2015
Same author

The future of set (single embryo transfer) in the United States.

Facts, views & vision in ObGyn·2014

Area of Science:

  • Oncology
  • Surgical Pathology
  • Medical Imaging

Background:

  • Vulvar carcinoma diagnosis and staging rely on accurate assessment of lymph node involvement.
  • Sentinel lymph node detection (SLND) is critical for staging and guiding treatment in vulvar cancer.
  • Microstaging techniques can improve the sensitivity of detecting minimal metastatic disease.

Purpose of the Study:

  • To evaluate the efficacy of combined complementary techniques for sentinel lymph node (SLN) detection in vulvar carcinoma.
  • To assess the utility of microstaging for identifying micrometastases in SLNs.

Main Methods:

  • Patients with invasive vulvar carcinoma underwent SLND using lymphoscintigraphy, isosulfan blue dye, and a gamma-detecting probe.
  • Sentinel nodes were examined using hematoxylin and eosin (H&E) staining.

Related Experiment Videos

  • Pathologically negative SLNs were further analyzed with serial sectioning and immunohistochemistry for cytokeratin.

    • Main Results:

    • Combined-modality mapping achieved high SLN detection rates.
    • Isosulfan blue dye identified an additional SLN in two cases.
    • Histologic microstaging detected micrometastases in 11% of initially negative SLNs.

    Conclusions:

    • Combined-modality mapping significantly enhances SLN detection in vulvar carcinoma.
    • Histologic microstaging is essential for improving the detection of micrometastases within SLNs, refining cancer staging.