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Comet Assay as an Indirect Measure of Systemic Oxidative Stress
Published on: May 22, 2015
Laparoscopic surgery-induced changes in oxidative stress markers in human plasma
G K Glantzounis1, A D Tselepis, A P Tambaki
1Department of Surgery, G. Hatzikosta General Hospital, Makriyianni Avenue, GR45001 Ioannina, Greece.
Surgical Endoscopy
|December 1, 2001
Summary
Laparoscopic surgery causes free radical damage and decreased antioxidants, impacting liver function. This suggests a human ischemia-reperfusion model from pneumoperitoneum inflation and deflation.
Area of Science:
- Surgical Physiology
- Biochemistry
Background:
- Pneumoperitoneum induction in laparoscopic surgery increases intraabdominal pressure, leading to splanchnic ischemia.
- Deflation normalizes intraabdominal pressure and splanchnic blood flow, suggesting an ischemia-reperfusion model in humans.
Purpose of the Study:
- To investigate the effects of laparoscopic cholecystectomy (LC) on oxidative stress and liver function.
- To compare these effects with open cholecystectomy (OC).
Main Methods:
- Thirty patients undergoing LC and 20 undergoing OC were studied.
- Plasma levels of thiobarbituric acid-reactive substances (TBARS), total antioxidant status (TAS), and uric acid (UA) were measured.
- Liver function tests (AST, ALT, TBL) were assessed preoperatively and postoperatively.
Main Results:
- LC significantly increased TBARS and decreased TAS and UA postoperatively.
- LC also led to significant increases in AST, ALT, and TBL.
- OC showed no significant changes in TBARS, with similar alterations in other parameters as LC.
Conclusions:
- Laparoscopic surgery, particularly pneumoperitoneum deflation, induces free radical-generated lipid peroxidation.
- This is associated with reduced antioxidant capacity and altered liver function, indicative of ischemia-reperfusion injury.
