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Induction of bacteriolytic enzyme from pyocinogenic Pseudomonas aeruginosa and its enzymatic properties
Abstract:
Mitomycin C induced a pyocinogenic Pseudomonas aeruginosa P15 to produce a bacteriolytic enzyme, PR1-lysozyme, together with pyocin R1. No significant accumulation of the enzyme was observed inside the induced cells. The enzyme was partially purified by acrinol treatment and Amberlie CG-50 column chromatography. The mode of action of the enzyme on the host bacterial cells as well as on Micrococcus lysodeikticus cells or peptidoglycan isolated from Salmonella typhimurium, was compared with that of hen egg-white lysozyme or phage lambda-lysozyme. It is suggested that PR1-lysozyme should be classified as a glycosidase, rather than an amidase or an endopeptidase.
Insights
Mitomycin C induction of Pseudomonas aeruginosa P15 yielded PR1-lysozyme, a bacteriolytic enzyme. This enzyme acts as a glycosidase, breaking down bacterial cell walls.
Area of Science:
- Microbiology
- Enzymology
Background:
- Pseudomonas aeruginosa is a pyocinogenic bacterium.
- Bacteriolytic enzymes play a role in controlling bacterial populations.
Purpose of the Study:
- To characterize the bacteriolytic enzyme PR1-lysozyme produced by Pseudomonas aeruginosa P15.
- To compare the mode of action of PR1-lysozyme with other known lysozymes.
Main Methods:
- Induction of Pseudomonas aeruginosa P15 with Mitomycin C.
- Partial purification of PR1-lysozyme using acrinol treatment and ion-exchange chromatography.
- Comparative analysis of enzyme activity on bacterial cells, isolated peptidoglycan, and Micrococcus lysodeikticus.
Main Results:
- Mitomycin C induction resulted in the production of PR1-lysozyme and pyocin R1.
- PR1-lysozyme was extracellularly released, with no significant intracellular accumulation.
- PR1-lysozyme demonstrated lytic activity comparable to hen egg-white lysozyme and phage lambda-lysozyme.
Conclusions:
- PR1-lysozyme is a bacteriolytic enzyme produced by Pseudomonas aeruginosa.
- The enzyme's mode of action suggests it functions as a glycosidase.
- PR1-lysozyme should be classified as a glycosidase, distinct from amidases or endopeptidases.