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Related Experiment Videos

In vivo imaging of light-emitting probes.

B W Rice1, M D Cable, M B Nelson

  • 1Xenogen Corporation, 860 Atlantic Avenue, Alameda, CA 94501, USA. brice@xenogen.com

Journal of Biomedical Optics
|December 1, 2001
PubMed
Summary

In vivo imaging uses light-emitting probes in research animals. Red to infrared light reporters and sensitive CCD cameras enable detection of small cell populations deep within tissues.

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Area of Science:

  • Biomedical imaging
  • Molecular imaging
  • Small animal imaging

Background:

  • In vivo imaging with light-emitting probes is vital for biological studies in research animals.
  • Reporters emitting red to infrared light (>600 nm) are optimal due to low tissue absorption.
  • Detecting bioluminescent signals depends on cell count and tissue depth.

Purpose of the Study:

  • To describe instrumentation for in vivo imaging.
  • To present examples of bioluminescent cell imaging in mice.
  • To evaluate reporter probes and imaging devices for in vivo applications.

Main Methods:

  • Utilized firefly luciferase and fluorescent proteins as light-emitting probes.
  • Employed photon diffusion modeling to estimate detection limits.
  • Compared cooled back-thinned integrating CCDs with image-intensified CCDs.
  • Developed and presented Xenogen in vivo imaging instrumentation.

Main Results:

  • Bioluminescent cell counts as low as a few hundred are detectable subcutaneously.
  • Approximately 10(6) cells are needed for detection at 2 cm depth.
  • Cooled back-thinned integrating CCDs offer superior performance (>600 nm) due to high quantum efficiency.
  • Demonstrated successful in vivo imaging of bioluminescent cells in mice.

Conclusions:

  • In vivo imaging with red/infrared light-emitting probes and sensitive CCDs is effective for biological research.
  • Instrumentation advancements facilitate sensitive detection of small cell populations in vivo.
  • The choice of reporter and detector is critical for successful deep-tissue imaging.

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