Related Experiment Videos
Arginine inhibits hemostasis activation.
T W Stief1, M Weippert, V Kretschmer
1Department of Clinical Chemistry and Molecular Diagnostics, Hospital of Philipps University, D-35033 Marburg, Germany. thstief@post.med.uni-marburg.de
Thrombosis Research
|December 1, 2001
Summary
L-arginine effectively inhibits in vitro hemostasis activation, showing potential as a stabilizer for blood samples and a therapeutic agent for coagulation disorders. This amino acid offers a promising approach to managing hemostasis activation in clinical settings.
Area of Science:
- Biochemistry
- Hematology
- Pharmacology
Background:
- In vivo hemostasis activation requires precise diagnosis and therapy.
- In vitro hemostasis (coagulation and fibrinolysis) must be prevented from artefactual changes.
- Standard anticoagulation with sodium citrate is insufficient for complete inhibition of hemostasis activation.
Purpose of the Study:
- To investigate L-arginine and guanidine as potential physiologic inhibitors of hemostasis activation.
- To evaluate their efficacy in preventing in vitro coagulation and fibrinolysis.
- To explore their utility in both diagnostic and therapeutic applications for hemostasis disorders.
Main Methods:
- Assessed whole blood clotting time (WBCT), prothrombin time (PT), activated partial thromboplastin time (APTT), and in vitro bleeding test closure time (IVBT-CT).
- Utilized whole blood aggregometry (WBA) to measure platelet function.
- Tested varying concentrations of L-arginine and guanidine in normal human blood and plasma.
Main Results:
- L-arginine demonstrated dose-dependent inhibition of WBCT, PT, APTT, IVBT-CT, and WBA.
- A concentration of 50 mM L-arginine significantly prolonged clotting times and inhibited platelet aggregation.
- L-arginine exhibited superior anti-epinephrine action compared to anti-ADP action.
Conclusions:
- L-arginine and guanidine effectively inhibit hemostasis activation in vitro.
- L-arginine shows promise as a hemostasis stabilizer for diagnostic and therapeutic use.
- Potential applications include improving blood product storage and managing conditions like disseminated intravascular coagulation (DIC).