Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Pyrogen testing in vitro using the Limulus test].

D Krüger, G Zimmermann, G Hebold

    Arzneimittel-Forschung
    |February 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Localizing value of cutaneous ictal phenomena: A systematic review.

    Epileptic disorders : international epilepsy journal with videotape·2026
    Same author

    Post-colonoscopy colorectal cancers in privately insured patients in South Africa.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2025
    Same author

    Response to: Post-colonoscopy colorectal cancer incidence.

    South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2025
    Same author

    Biochemical markers in emergency thoracic surgery in penetrating trauma.

    South African journal of surgery. Suid-Afrikaanse tydskrif vir chirurgie·2024
    Same author

    Contribution to the Pathological Physiology of Pneumonia.

    Medical examiner (Philadelphia, Pa.)·2023
    Same author

    Microgravity studies of solidification patterns in model transparent alloys onboard the International Space Station.

    NPJ microgravity·2023

    The Limulus test, an in vitro method, shows promise for pyrogen detection, proving more sensitive than rabbit testing in some cases. It can complement traditional pyrogen testing for specific applications.

    Area of Science:

    • Microbiology
    • Pharmacology
    • Toxicology

    Background:

    • Pyrogens pose a significant risk in pharmaceutical products.
    • The rabbit pyrogen test is a traditional method for detecting pyrogens.
    • The Limulus amebocyte lysate (LAL) test offers an alternative in vitro method.

    Purpose of the Study:

    • To evaluate the applicability of the Limulus test compared to the rabbit pyrogen test.
    • To assess the sensitivity of the Limulus test against bacterial strains.

    Main Methods:

    • Comparative analysis of Limulus test and rabbit pyrogen test results.
    • Testing of raw materials and drug lots for pyrogens.
    • Sensitivity testing using various bacterial strains at modified concentrations.

    Related Experiment Videos

    Main Results:

    • Pyrogens were detected in 6 out of 24 lots using the rabbit pyrogen test.
    • 2 of these 6 lots yielded positive results with the Limulus test, with no false positives.
    • The Limulus test demonstrated higher sensitivity than the rabbit test when examining 7 bacterial strains.

    Conclusions:

    • The Limulus test is a viable alternative and potential complement to the rabbit pyrogen test.
    • Its in vitro nature and sensitivity make it suitable for specific pyrogen detection scenarios.
    • Further investigation into its complementary role in addressing specific challenges in pyrogen testing is warranted.