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Published on: September 30, 2014
Proteins of the lactococcin A secretion system: lcnD encodes two in-frame proteins
M Varcamonti1, G Nicastro, G Venema
1Biomolecular Sciences and Biotechnology Institute, Department of Genetics, University of Groningen, Haren, The Netherlands. varcamon@unina.it
Abstract:
Polyclonal antibodies were raised against LcnC and LcnD proteins of the Lactococcus lactis bacteriocin lactococcin A secretory system to examine their cellular location and interaction. Two major reacting bands were detected by Western immunoblot with the anti-LcnD antibody: one of 52 kDa (LcnD) and another of 45 kDa, called here LcnD*. LcnD* was still detectable after removing the AUG start codon for LcnD. Chemical cross-linking analyses of membrane fractions of L. lactis cells expressing the LcnC/D secretion machinery were performed. Our results indicate that LcnD is present in the secretion machinery complex as a dimer and is able to interact with LcnD* and LcnC.
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