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Related Experiment Videos

SLC19A3 encodes a second thiamine transporter ThTr2.

A Rajgopal1, A Edmondnson, I D Goldman

  • 1Departments of Medicine and Molecular Pharmacology, Albert Einstein College of Medicine Cancer Center, Chanin 628, 1300 Morris Park Avenue, Bronx, NY 10461, USA.

Biochimica Et Biophysica Acta
|December 4, 2001
PubMed
Summary
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Researchers identified SLC19A3 as a second thiamine transporter (ThTr2). This facilitative carrier specifically transports vitamin B1, distinct from folate transporters in the same family.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Nutritional Science

Background:

  • A family of facilitative carriers includes reduced folate (SLC19A1) and thiamine (SLC19A2) transporters.
  • Despite sequence similarity, SLC19A1 and SLC19A2 exhibit distinct substrate specificities, transporting folates and thiamine, respectively.
  • The functional roles of newly identified members within this transporter family require elucidation.

Purpose of the Study:

  • To characterize the function of the recently cloned SLC19A3 transporter.
  • To determine the substrate specificity and kinetic properties of SLC19A3.
  • To ascertain whether SLC19A3 represents a novel thiamine transporter.

Main Methods:

  • Transient transfection of SLC19A3 cDNA into HeLa cells.

Related Experiment Videos

  • Measurement of [3H]thiamine, methotrexate, and folic acid uptake in transfectant cells.
  • Analysis of thiamine transport activity across a range of pH values.
  • Inhibition studies using unlabeled thiamine and other organic cations.

    • Main Results:

    • SLC19A3 transfectants showed significantly enhanced [3H]thiamine uptake compared to vector controls.
    • Methotrexate and folic acid uptake were not increased in SLC19A3 transfectants, indicating substrate specificity.
    • Thiamine transport activity exhibited a pH optimum around 7.5.
    • Uptake of [3H]thiamine was specifically inhibited by unlabeled thiamine but not by other organic cations.

    Conclusions:

    • SLC19A3 functions as a high-affinity, specific transporter for thiamine (vitamin B1).
    • The characteristics of SLC19A3 align with those of a second thiamine transporter, designated ThTr2.
    • This finding expands the known repertoire of thiamine transporters within the SLC19 family.