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Related Experiment Videos

Advanced integrated mouse YAC map including BAC framework.

L C Schalkwyk1, B Cusack, I Dunkel

  • 1Max-Planck-Institute of Molecular Genetics, Ihnestrasse 73, D-14195 Berlin, Germany. L.Schalkwyk@iop.kcl.ac.uk

Genome Research
|December 4, 2001
PubMed
Summary
This summary is machine-generated.

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Researchers developed an advanced Yeast Artificial Chromosome (YAC) contig map for the mouse genome, significantly increasing clone depth and marker density. This comprehensive map includes Bacterial Artificial Chromosome (BAC) clones, facilitating positional cloning and genomic sequencing efforts.

Area of Science:

  • Genomics
  • Mouse genetics

Background:

  • Comprehensive physical maps are essential for mouse genome functional characterization and positional cloning.
  • Creating contigs of genetically defined regions is a critical, often challenging, step in positional cloning.
  • Yeast Artificial Chromosome (YAC) clones are vital for constructing these contigs.

Purpose of the Study:

  • To generate an advanced, second-generation YAC contig map of the mouse genome.
  • To increase clone depth and marker density for improved molecular access to genomic regions.
  • To create a dense framework of Bacterial Artificial Chromosome (BAC) clones for genomic sequencing.

Main Methods:

  • Utilized Interspersed Repetitive Sequence (IRS)-PCR genomics.
  • Integrated data from four large-insert mouse YAC libraries across three strains.

Related Experiment Videos

  • Incorporated existing data from Hunter et al. and Nusbaum et al.

    • Main Results:

    • Developed a YAC contig map with doubled clone depth and marker density.
    • Mapped 1942 Bacterial Artificial Chromosome (BAC) clones, creating a genome-spanning framework.
    • The final map contains 20,205 markers and 56,093 YACs.

    Conclusions:

    • The enhanced YAC contig map provides a robust tool for mouse genome research.
    • The integrated BAC framework serves as a foundation for genomic sequencing projects.
    • This resource significantly advances the ability to link genotype to phenotype in mice.