Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The STRO-1+ marrow cell population is multipotential.

James E Dennis1, Jean-Pierre Carbillet, Arnold I Caplan

  • 1Skeletal Research Center, Department of Biology, Case Western Reserve University, Cleveland, Ohio 44106, USA. jed4@po.cwru.edu

Cells, Tissues, Organs
|December 4, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Gene coexpression networks: concepts and applications].

Biologie aujourd'hui·2025
Same author

Culture Expansion Alters Human Bone Marrow-Derived Mesenchymal Stem Cell Production of Osteoarthritis-Relevant Cytokines and Growth Factors.

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North America and the International Arthroscopy Association·2024
Same author

Multidisciplinary Management Strategies for Long COVID: A Narrative Review.

Cureus·2024
Same author

Amino Acid Uptake Limitations during Human Mesenchymal Stem Cell-Based Chondrogenesis.

Tissue engineering. Part A·2024
Same author

A multistep computational approach reveals a neuro-mesenchymal cell population in the embryonic hematopoietic stem cell niche.

Development (Cambridge, England)·2024
Same author

MSCs mediate long-term efficacy in a Crohn's disease model by sustained anti-inflammatory macrophage programming via efferocytosis.

NPJ Regenerative medicine·2024

Human STRO-1+ cells from bone marrow can transform into various cell types. These cells support blood formation and can become fat, bone, and cartilage cells, demonstrating mesenchymal stem cell potential.

Area of Science:

  • Stem Cell Biology
  • Mesenchymal Stem Cell Differentiation
  • Hematopoiesis

Background:

  • Human bone marrow contains cells with stem cell properties.
  • STRO-1 is an antigen expressed on a subset of these cells.
  • Understanding STRO-1+ cell potential is crucial for regenerative medicine.

Purpose of the Study:

  • To investigate the differentiation potential of human marrow-derived STRO-1+ cells.
  • To determine if STRO-1+ cells can differentiate into multiple mesenchymal lineages.
  • To assess the role of STRO-1+ cells in supporting hematopoiesis.

Main Methods:

  • Isolation of STRO-1+ cells using magnetic immunobeads.
  • Co-culture of STRO-1+ cells with hematopoietic precursors.
  • Induction of differentiation into adipocytic, osteogenic, and chondrogenic lineages.

Related Experiment Videos

  • In vitro and in vivo assays for differentiation potential.

    • Main Results:

    • STRO-1+ cells supported hematopoietic progenitor growth and cobblestone area formation.
    • Hematopoiesis-supportive stromal cells exhibited myofibroblastic and adipocytic phenotypes.
    • STRO-1+ cells differentiated into adipocytes, osteoblasts (forming bone in vivo), and chondrocytes (expressing type II and X collagens).

    Conclusions:

    • Human marrow-derived STRO-1+ cells possess multipotent mesenchymal differentiation capacity.
    • STRO-1+ cells can differentiate into adipocytes, osteoblasts, and chondrocytes.
    • These cells also function as hematopoiesis-supportive stromal cells with a vascular smooth muscle-like phenotype.