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Related Concept Videos

Protein Complexes with Interchangeable Parts01:57

Protein Complexes with Interchangeable Parts

Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
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Sec61 protein conducting channel
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Sgf1p, a new component of the Sec34p/Sec35p complex.

D W Kim1, T Massey, M Sacher

  • 1Department of Cell Biology and Howard Hughes Medical Institute, Yale University, New Haven, CT, USA.

Traffic (Copenhagen, Denmark)
|December 6, 2001
PubMed
Summary

We identified SGF1 as a suppressor of sec35-1 mutants. SGF1 encodes a new subunit of the Sec34p/Sec35p complex, crucial for endoplasmic reticulum to Golgi traffic and protein secretion.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Protein Trafficking

Background:

  • The Sec34p/Sec35p complex is involved in intracellular transport.
  • Mutations in SEC35 cause defects in protein trafficking.

Purpose of the Study:

  • To identify genes that suppress mutations in SEC35.
  • To characterize the function of the novel suppressor gene SGF1.
  • To elucidate the role of SGF1 in the Sec34p/Sec35p complex and protein secretion.

Main Methods:

  • Yeast genetics and high-copy suppressor screening.
  • Yeast two-hybrid system and coprecipitation assays.
  • Immunofluorescence microscopy.
  • Analysis of protein secretion in mutant strains.

Main Results:

  • SGF1 was identified as a high-copy suppressor of the sec35-1 mutant.
  • SGF1 encodes an essential hydrophilic protein, Sgf1p, which is a novel subunit of the Sec34p/Sec35p complex.
  • Reduced Sgf1p levels cause membrane accumulation and block endoplasmic reticulum to Golgi traffic.
  • Sec34p localizes to the Golgi, particularly early Golgi regions.
  • Mutations in SEC34 and SEC35 result in a pleiotropic block of protein secretion.

Conclusions:

  • SGF1 is essential for the function of the Sec34p/Sec35p complex.
  • Sgf1p plays a critical role in endoplasmic reticulum to Golgi transport.
  • The Sec34p/Sec35p complex, including Sgf1p, is required for efficient protein secretion.