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Rice straw as a support for immobilization of microbial lipase
H F de Castro1, R de Lima, I C Roberto
1Department of Chemical Engineering and Department of Biotechnology, Faculty of Chemical Engineering of Lorena, P.O. Box 116, 12600-970 Lorena, SP, Brazil. heizir@dequi.faenquil.br
Abstract:
Candida rugosa lipase was covalently immobilized on rice straw activated with glutaraldehyde using poly(ethylene glycol) (PEG) as the stabilizing agent. The effects of PEG molecular weight and enzyme loading were studied according to a full 2(2) factorial design. Higher immobilization yields (>70%) were attained when the lipase loading was 95 units/mg of dry support, independent of PEG molecular weight. All derivatives showed high hydrolytic and synthetic activities. This work provides preliminary results on the use of agricultural residues as a support matrix for immobilizing lipase and on the application of the resulting derivatives to butyl butyrate synthesis as a study model.