Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluidized bed design parameters affecting novel lactic Acid downstream processing.

A V Sosa1, P R Córdoba, N I Perotti

  • 1PROIMI-Biotecnología, Av. Belgrano y Pje. Caseros, (4000) SM Tucumán, Argentina. anasosa@uol.com.br

Biotechnology Progress
|December 12, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Design and characterization of immobilized biocatalyst with lipase activity onto magnetic magnesium spinel nanoparticles: A novel platform for biocatalysis.

Colloids and surfaces. B, Biointerfaces·2018
Same author

Designing cross-linked xylanase aggregates for bioconversion of agroindustrial waste biomass towards potential production of nutraceuticals.

International journal of biological macromolecules·2018
Same author

Two amplicon sequencing strategies revealed different facets of the prokaryotic community associated with the anaerobic treatment of vinasses from ethanol distilleries.

Bioresource technology·2014
Same author

pH control of the production of recombinant glucose oxidase in Aspergillus nidulans.

Journal of applied microbiology·2004
Same author

Modeling of direct recovery of lactic acid from whole broths by ion exchange adsorption.

Bioseparation·2001
Same author

Continuous production of L(+)-lactic acid by Lactobacillus casei in two-stage systems.

Applied microbiology and biotechnology·1999

Increasing fluidized bed column height significantly boosts lactic acid binding capacity by 50%. This optimization enhances resin contact time for improved purification from fermentation broths.

Area of Science:

  • Biochemical Engineering
  • Separation Science
  • Industrial Biotechnology

Background:

  • Lactic acid is a key platform chemical produced via fermentation.
  • Efficient purification is crucial for its industrial applications.
  • Fluidized bed chromatography offers potential for continuous lactic acid recovery.

Purpose of the Study:

  • To investigate the impact of column diameter and bed height on lactic acid binding and elution.
  • To optimize resin performance for lactic acid purification.
  • To understand the relationship between design parameters and purification efficiency.

Main Methods:

  • Purification of lactic acid directly from fermentation broth.
  • Utilized a fluidized bed column packed with strong anionic exchange resin.

Related Experiment Videos

  • Varied settled bed height (2.5 to 5 cm) and column diameter.

    • Main Results:

    • A 50% increase in resin binding capacity was achieved by increasing settled bed height.
    • This enhancement was observed across all tested column diameters.
    • Increased binding capacity correlated with higher resin contact time and residence time.

    Conclusions:

    • Fluidized bed column height is a critical parameter for optimizing lactic acid binding capacity.
    • Increased bed height enhances purification efficiency through improved resin-broth interaction.
    • This study provides valuable insights for designing efficient lactic acid purification systems.