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Detection of cytochrome P450 mRNA transcripts in prostate samples by RT-PCR
N Finnström1, C Bjelfman, T G Söderström
1Department of Medical Laboratory Sciences and Technology, Clinical Pharmacology, Karolinska Institutet at Huddinge University Hospital, S-141 86 Stockholm, Sweden. niklas.finnstrom@labtek.ki.se
Background:
The expression of cytochrome P450 (CYP)-dependent mono-oxygenases in the prostate is important, as it will determine the rate of activation of potential carcinogens as well as the metabolism of hormones with implications in diseases of the prostate. In addition, the levels of cytochromes P450 in prostatic tumours may well be determinants of the outcome of therapy involving P450 substrates such as anti-androgens.
Methods:
The gene expression of 12 different CYP genes was measured by reverse transcription-polymerase chain reaction (RT-PCR) in a total of 28 human prostatic tumour and nontumour samples.
Results:
Intriguingly, a large number of CYP mRNAs were detected in the prostate samples, including CYP1A2, -1B1, -2C19, -2D6, -3A4, -3A5, -3A7 and -4B1. CYP1B1 was consistently expressed and CYP3A5 and CYP4B1 were expressed in a majority of the samples tested.
Conclusions:
These data demonstrate a wide range of CYP genes being expressed in the prostate. The relative importance of these enzymes in the pathogenesis and treatment of prostatic disease remains an important theme for further study.