Related Experiment Videos
A rapid and sensitive method for the detection of Yersinia enterocolitica strains from clinical samples
H M Hussein1, S G Fenwick, J S Lumsden
1Institute of Veterinary, Animal and Biomedical Sciences, College of Science, Massey University, Palmerston North, New Zealand.
Aims:
To compare three culture methods to detect Yersinia enterocolitica from oral or rectal swabs from experimentally infected pigs.
Methods And Results:
The three methods used were: direct plating on Cefsulodin-Irgasan-Novobiocin (CIN) agar, cold enrichment in phosphate buffered saline (PBS) followed by plating on CIN agar and selective enrichment with Luria-Bertani-Bile Salts Irgasan (LB-BSI) followed by plating on CIN agar. Selective enrichment with LB-BSI produced the highest recovery rate (63%), when compared with cold enrichment (52%) and plating on CIN agar alone (43%). Selective enrichment with LB-BSI was significantly (P < 0.02) more sensitive than direct plating on CIN agar and more sensitive than cold enrichment (P < 0.1).
Conclusions, Significance And Impact Of The Study:
Selective enrichment with LB-BSI was more sensitive than the widely accepted method of cold enrichment and it reduced the time required for detection of Y. enterocolitica by three weeks. Selective enrichment with LB-BSI was also compatible with a multiplex PCR technique.