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Harvesting Venom Toxins from Assassin Bugs and Other Heteropteran Insects
Published on: April 21, 2018
Isolation of the major lethal toxin in the venom of Bungarus flaviceps
O Khow1, L Chanhome, T Omori-Satoh
1Queen Saovabha Memorial Institute, Thai Red Cross Society, 1871 Rama IV Road, 10330, Bangkok, Thailand.
Abstract:
The major lethal toxin in the venom of Bungarus flaviceps has been isolated by ion-exchange chromatography, absorption chromatography and RP-HPLC with a 14-fold purification and an overall yield of 16.5% of the lethal toxicity contained in crude venom. Its sublethal dose (LD(50)) determined in mice weighing 18-20 g was 0.25 (0.19-0.32) microg per mouse. The lethal toxin was pure according to disc- and SDS-PAGE as well as gel HPLC. Its apparent molecular weight determined by SDS-PAGE was 29 kDa. It is a basic protein consisting of two polypeptide chains having apparent molecular weights of 17 and 8 kDa, respectively. The toxin has PLA activity but is free of ACE activity.
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