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Related Experiment Videos

Do sex steroids regulate glutamine synthesis with age?

Lionel Verdier1, Yves Boirie, Sebastien Van Drieesche

  • 1Unité de Nutrition et Métabolisme Protéique, Centre de Recherche en Nutrition Humaine de Clermont-Ferrand, and Institut National de la Recherche Agronomique, 63122 Ceyrat, France.

American Journal of Physiology. Endocrinology and Metabolism
|December 12, 2001
PubMed
Summary

Glucocorticoids regulate glutamine synthetase (GS) in female rat muscle. Sex steroids like progesterone and estradiol do not influence GS activity with aging, suggesting reproductive status doesn't impact muscle GS.

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Area of Science:

  • Endocrinology
  • Muscle Physiology
  • Aging Research

Background:

  • Glutamine synthetase (GS) is crucial for glutamine synthesis.
  • GS is induced by glucocorticoids and maintained during aging in skeletal muscle.
  • The role of sex steroids (progesterone, estradiol) in regulating muscle GS during aging is unknown.

Purpose of the Study:

  • To investigate the role of progesterone and estradiol in regulating glutamine synthetase (GS) with aging in female rats.
  • To determine if sex steroids influence muscle GS activity and mRNA expression in young and aged female rats.

Main Methods:

  • Adult and aged female Wistar rats underwent adrenalectomy (ADX), ovariectomy (OV), or both (ADXOV), compared to sham controls.
  • Muscle GS activity and mRNA expression in tibialis anterior were measured.

Related Experiment Videos

  • Hormone replacement therapy with progesterone and estradiol was assessed.

    • Main Results:

    • Adrenalectomy significantly decreased muscle GS activity and expression in both age groups.
    • Ovariectomy had no effect on muscle GS activity at any age.
    • Progesterone and estradiol replacement did not restore muscle GS response after ADX or OV.
    • Heart GS activity decreased with ADX only in aged rats.

    Conclusions:

    • Reproductive endocrine status does not affect skeletal muscle glutamine synthetase activity in female rats.
    • Aging may alter the regulation of heart glutamine synthetase by steroids.