Chromosome painting - principles, strategies and scope

Chromosome Painting is emerging as a powerful tool in the exact localization of different gene sequences of chromosomes at the microscopic level. It is principally based on molecular hybridization in situ with sequence specific probes on chromosomes. Different strategies have been adopted for the preparation of probes, hybridization and visualization. The impact of this method lies in identification of genes for desired characters in the chromosomes, including those of genetic disorders, in cancer research, in transgenesis and in studies on biodiversity and evolution.