Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

High-resolution scanner for neuroanatomical analysis.

Karl E Krout1, John M Jenkins, Arthur D Loewy

  • 1Department of Anatomy and Neurobiology, Washington University School of Medicine, 660 South Euclid Ave., Box 8108, St. Louis, MO 63110, USA. kekrout@pcg.wustl.edu

Journal of Neuroscience Methods
|December 14, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Contralateral Limb Specificity for Movement Preparation in the Parietal Reach Region.

The Journal of neuroscience : the official journal of the Society for Neuroscience·2022
Same author

Despite increasing aldosterone, elevated potassium is not necessary for activating aldosterone-sensitive HSD2 neurons or sodium appetite.

Physiological reports·2021
Same author

CNS sites activated by renal pelvic epithelial sodium channels (ENaCs) in response to hypertonic saline in awake rats.

Autonomic neuroscience : basic & clinical·2016
Same author

Commentary on: Saper CB, Loewy AD, Swanson LW, Cowan WM. (1976) Direct hypothalamo-autonomic connections. Brain Research 117:305-312.

Brain research·2016
Same author

Commentary on: Efferent connections of the parabrachial nucleus in the rat. C.B. Saper and A.D. Loewy, Brain Research 197:291-317, 1980.

Brain research·2016
Same author

Blockade of ENaCs by amiloride induces c-Fos activation of the area postrema.

Brain research·2015

A high-resolution flatbed scanner enables detailed neuroanatomical studies by visualizing labeled neurons alongside cytoarchitectonic structures. This automated method accelerates brain mapping and analysis, offering broad applications for cell distribution evaluation.

Area of Science:

  • Neuroscience
  • Anatomical Imaging
  • Histology

Background:

  • Accurate neuroanatomical analysis requires precise visualization of cellular structures and their spatial relationships.
  • Traditional methods for mapping brain sections are time-consuming and may lack simultaneous cellular and structural context.

Purpose of the Study:

  • To introduce and evaluate a high-resolution flatbed scanning method for neuroanatomical studies.
  • To demonstrate the ability to visualize labeled neurons and cytoarchitectonic structures concurrently.
  • To assess the efficiency and potential applications of this novel imaging technique.

Main Methods:

  • Utilizing a high-resolution flatbed scanner to image thionin-stained rat brain sections labeled with gold intensified diaminobenzidine (DAB).

Related Experiment Videos

  • Generating high-resolution maps of complete rat brains (approx. 100 sections at 250 microm intervals).
  • Automating scanning processes to increase speed compared to conventional methods.

    • Main Results:

    • Successful resolution of individual neurons and their precise location relative to cytoarchitectonic features.
    • Generation of comprehensive rat brain maps within 10 hours.
    • Demonstration of a 50% speed increase over traditional mapping techniques.

    Conclusions:

    • High-resolution flatbed scanning offers a rapid and accurate method for neuroanatomical analysis.
    • The technique facilitates simultaneous visualization of labeled cells and tissue architecture.
    • Potential applications include imaging Fos-immunoreactive neurons, reporter gene expression, and pathological inclusions like Alzheimer's plaques without microscopy.