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Related Experiment Videos

STR primer concordance study.

B Budowle1, A Masibay, S J Anderson

  • 1FBI, Laboratory Division, 935 Pennsylvania Avenue, NW, Washington, DC 20535, USA. bbudowle@fbi.gov

Forensic Science International
|December 14, 2001
PubMed
Summary

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This summary is machine-generated.

Forensic DNA typing kits, PowerPlex 16 and Profiler Plus/COfiler, demonstrate reliability for CODIS use across diverse populations. Allele dropout was minimal, and new databases for STR loci Penta D and Penta E were established.

Area of Science:

  • Forensic Genetics
  • Population Genetics
  • Molecular Biology

Background:

  • Accurate DNA typing is crucial for forensic applications, including the Combined DNA Index System (CODIS).
  • Establishing population-specific allele frequency databases is essential for interpreting forensic DNA evidence.
  • Short tandem repeat (STR) loci are widely used in forensic DNA profiling due to their high polymorphism.

Purpose of the Study:

  • To evaluate the reliability of PowerPlex 16 and Profiler Plus/COfiler kits for DNA typing across diverse populations.
  • To identify potential issues like allele dropout in specific populations and loci.
  • To establish allele frequency databases for the Penta D and Penta E STR loci.

Main Methods:

  • DNA samples from over 1500 individuals representing African Americans, Caucasians, Hispanics, Native Americans, Chamorros, and Filipinos were analyzed.

Related Experiment Videos

  • Typing was performed using the PowerPlex 16 and Profiler Plus/COfiler amplification kits.
  • Allele frequencies for STR loci Penta D and Penta E were determined and databases were created.

    • Main Results:

    • The PowerPlex 16, Profiler Plus, and COfiler kits demonstrated high reliability for typing reference samples for CODIS.
    • Minor instances of allele dropout were observed, primarily at the D8S1179 locus in Chamorro and Filipino samples from Guam when using the Profiler Plus kit.
    • Comprehensive allele frequency databases for the highly polymorphic Penta D and Penta E STR loci were successfully established.

    Conclusions:

    • The tested STR typing kits are suitable for forensic applications and CODIS database use.
    • Awareness of potential allele dropout at specific loci in certain populations is important for accurate interpretation.
    • The newly established allele frequency databases for Penta D and Penta E enhance forensic analysis capabilities for diverse populations.