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Related Experiment Videos

p44/p42-MAP kinase expression in papillary thyroid carcinomas.

M C Specht1, C B Barden, T J Fahey

  • 1Department of Surgery, The New York Presbyterian Hospital-Weill Medical College of Cornell University, New York, NY 10021, USA.

Surgery
|December 14, 2001
PubMed
Summary

Mitogen-activated protein (MAP) kinase activation is common in papillary thyroid cancers (PTCs). Blocking this pathway with U0126 can inhibit thyroid cancer cell growth, suggesting it as a therapeutic target.

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Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Mitogen-activated protein (MAP) kinase signaling is implicated in human epithelial cancer growth and tumorigenesis.
  • Targeting the MAP kinase pathway may inhibit epithelial cancer progression.

Purpose of the Study:

  • To investigate MAP kinase activation in human papillary thyroid carcinomas (PTCs).
  • To analyze the expression of native MAP kinase (MAPK) and phosphorylated MAP kinase (pMAPK) in PTCs and thyroid cancer cell lines.

Main Methods:

  • Immunoblot analysis of MAPK and pMAPK in paired PTC specimens from 10 patients.
  • Analysis of MAPK expression and cell growth in 3 thyroid tumor cell lines treated with the MEK inhibitor U0126.

Main Results:

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  • MAPK expression was similar in PTCs and adjacent normal tissues.
  • Increased pMAPK expression was observed in 6 out of 10 PTC tumors compared to normal tissue.
  • U0126 treatment reduced pMAPK levels and serum-induced growth in all tested thyroid cancer cell lines.

Conclusions:

  • MAP kinase activation is a frequent event in PTCs.
  • MAP kinase signaling represents a potential therapeutic target for inhibiting PTC growth.