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Effect of lysophosphatidylcholine on expression of vascular endothelial growth factor in ECV304 cells
1Department of Pharmacology, School of Pharmacy, The Second Military Medical University, Shanghai 200433, China.
Aim:
To determine the effect of lysophosphatidylcholine (LPC) on the expression of vascular endothelial growth factor (VEGF) in human umbilical veins endothelial cell line (ECV304).
Methods:
VEGF protein production from ECV304 cells was determined by enzyme-linked immunosorbent assay (ELISA). VEGF protein and its receptor were also examined by immunohistochemical staining. After culturing for 24 h with LPC, VEGF mRNA expression in ECV304 was examined by in situ hybridization.
Results:
Cultured ECV304 cells could express VEGF receptor and a strong expression of VEGF receptor was induced by LPC 5 mg/L. LPC upregulated VEGF protein secreted from the ECV304 cells in a time- and concentration-dependent manner and LPC also stimulated VEGF mRNA expression in the ECV304 cells.
Conclusion:
LPC could induce a strong expression of VEGF in ECV304 cells.