Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The RecOR proteins modulate RecA protein function at 5' ends of single-stranded DNA.

J M Bork1, M M Cox, R B Inman

  • 1Department of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706-1544, USA.

The EMBO Journal
|December 18, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of the safety, reactogenicity and immunogenicity of FluBlok® trivalent recombinant baculovirus-expressed hemagglutinin influenza vaccine administered intramuscularly to healthy adults 50-64 years of age.

Vaccine·2011
Same author

Comparative immunogenicity of recombinant influenza hemagglutinin (rHA) and trivalent inactivated vaccine (TIV) among persons > or =65 years old.

Vaccine·2009
Same author

Neurobehavioral abnormalities in the dysbindin-1 mutant, sandy, on a C57BL/6J genetic background.

Genes, brain, and behavior·2009
Same author

CDH23 mutation and phenotype heterogeneity: a profile of 107 diverse families with Usher syndrome and nonsyndromic deafness.

American journal of human genetics·2002
Same author

Recombinational DNA repair of damaged replication forks in Escherichia coli: questions.

Annual review of genetics·2001
Same author

RecA protein filaments disassemble in the 5' to 3' direction on single-stranded DNA.

The Journal of biological chemistry·2001

The Escherichia coli RecF, RecO, and RecR proteins are key to DNA repair. RecOR proteins enhance RecA binding to DNA ends, facilitating repair more effectively than at DNA gaps.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • The Escherichia coli RecF, RecO, and RecR proteins are known to be involved in bacterial recombinational DNA repair, particularly at DNA gaps.
  • The RecA protein is central to DNA repair pathways, mediating strand exchange and filament formation.

Purpose of the Study:

  • To investigate the roles of Escherichia coli RecF, RecO, and RecR proteins in DNA repair dynamics.
  • To determine the influence of DNA structure (ends vs. gaps) on RecA protein binding facilitated by RecOR complex.

Main Methods:

  • In vitro biochemical assays were used to study protein-DNA interactions.
  • Analysis of RecA filament formation and stability in the presence of RecF, RecO, RecR, and single-stranded DNA (ssDNA) bound by single-stranded DNA-binding protein (SSB).

Related Experiment Videos

Main Results:

  • RecOR complex significantly accelerates RecA binding to linear ssDNA with DNA ends compared to ssDNA within gaps.
  • RecOR complex promotes RecA-mediated D-loop formation at the 5' ends of linear ssDNA.
  • RecR protein stabilizes RecA filaments, while RecF protein competes with RecO for RecR, destabilizing these filaments.

Conclusions:

  • Escherichia coli RecO and RecR proteins exhibit enhanced function at the 5' ends of linear ssDNA, facilitating RecA binding and stabilization.
  • The interplay between RecF, RecO, and RecR proteins regulates RecA filament dynamics during DNA repair, with DNA ends being preferential sites for RecOR-mediated enhancement.