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Related Experiment Videos

Phenotyping transgenic embryos: a rapid 3-D screening method based on episcopic fluorescence image capturing.

Wolfgang Johann Weninger1, Timothy Mohun

  • 1Integrative Morphology Group, Department of Anatomy, University of Vienna, Waehringerstrasse 13 A-1090 Wien, Austria. Wolfgang.Weninger@univie.ac.at

Nature Genetics
|December 18, 2001
PubMed
Summary

This study introduces Episcopic Fluorescence Image Capturing (EFIC) for 3-D embryo analysis. This method aids in studying cardiac development and gene expression in mouse models.

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Area of Science:

  • Developmental Biology
  • Morphology
  • Genetics

Background:

  • Three-dimensional (3-D) analysis of embryonic development is crucial for understanding morphology and disease.
  • Existing methods may lack the precision or ease required for routine analysis.

Purpose of the Study:

  • To present a novel technique for routine 3-D analysis of mouse embryos.
  • To demonstrate the utility of this technique in studying genetic mutations and gene expression patterns.

Main Methods:

  • Episcopic fluorescence image capturing (EFIC) of serial sections from wax-embedded mouse embryos.
  • Utilizing consistent and precisely aligned images for 3-D reconstruction.

Main Results:

  • Successfully applied EFIC to analyze the cardiac phenotype in trisomic 16 (Ts16) and Cited2-null mutant mouse embryos.

Related Experiment Videos

  • Visualized the expression pattern of an Myf5 enhancer/beta-galactosidase transgene.
  • Demonstrated the suitability of EFIC for 3-D analysis using animations and reconstruction software.

    • Conclusions:

    • EFIC is a simple and powerful tool for routine 3-D embryo and organ morphology analysis.
    • This technique is applicable to both normal and transgenic mouse models.
    • EFIC facilitates detailed investigation of developmental phenotypes and gene expression.