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Fluorescent Silver Staining of Proteins in Polyacrylamide Gels
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Fluorescent Silver Staining of Proteins in Polyacrylamide Gels

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A method for activity staining after native polyacrylamide gel electrophoresis using a coupled enzyme assay and

Jean Rivoal1, Christopher R Smith, Trevor F Moraes

  • 1Department of Plant Science, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada. rivoalj@ms.umanitoba.ca

Analytical Biochemistry
|December 18, 2001
PubMed

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Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...