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Related Experiment Videos

Experimental ASD closure using autologous cell-seeded interventional closure devices.

Christian Jux1, Harald Bertram, Peter Wohlsein

  • 1Department of Paediatric Cardiology and Paediatric Intensive Care Medicine, Hannover Medical School, Carl Neuberg Strasse 1, D-30627 Hannover, Germany.

Cardiovascular Research
|December 18, 2001
PubMed
Summary

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Autologous cell pre-seeding of atrial septal defect (ASD) occluders shows promise for faster tissue integration. While cells survive implantation stress, increased thrombogenicity currently limits in vivo application of these biodegradable devices.

Area of Science:

  • Biomaterials Science
  • Cardiovascular Surgery
  • Tissue Engineering

Background:

  • Atrial septal defect (ASD) occluders are permanent implants requiring tissue integration.
  • Current occluders pose long-term risks due to non-degradable foreign material.
  • Biodegradable devices need rapid, firm, and complete incorporation.

Purpose of the Study:

  • To investigate the feasibility of autologous cell-seeded ASD occluders.
  • To test the effect of collagen coating on cellular stress resistance in vitro.
  • To compare short-term in vivo effects of cell-seeded, collagen-coated, and acellular occluders.

Main Methods:

  • Autologous fibroblasts were used to pre-seed native and collagen-coated Dacron and Starflex devices.
  • In vitro mechanical stress tests were performed.

Related Experiment Videos

  • A sheep model was used to compare pre-seeded and conventional acellular Starflex devices in vivo.

    • Main Results:

    • Successful pre-seeding of ASD closure devices with autologous cells was achieved.
    • Collagen coating improved cellular density and mechanical stability after implantation.
    • Pre-seeded devices showed significantly thicker granulation tissue ingrowth compared to conventional occluders after 30 days.

    Conclusions:

    • Autologous cell pre-seeding is feasible, with cells surviving implantation stress.
    • Enhanced tissue ingrowth was observed with pre-seeded, collagen-coated devices.
    • Increased thrombogenicity remains a limitation for current in vivo application.