Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Delayed expression of the NFH subunit in differentiating P19 cells.

S J Pyle1, K G Roberts, K R Reuhl

  • 1Biology Department, University of North Dakota, P.O. Box 9019, Grand Forks, ND 58202-9019, USA. sally_pyle@und.nodak.edu

Brain Research. Developmental Brain Research
|December 18, 2001
PubMed
Summary

Retinoic acid (RA) induces neural differentiation in P19 cells. This study found high molecular weight neurofilament (NFH) expression, which occurs later than other neurofilament subunits during this neural development process.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced Risk Stratification for Children and Young Adults with B-Cell Acute Lymphoblastic Leukemia: A Children's Oncology Group Report.

Leukemia·2024
Same author

MLL rearrangements impact outcome in HOXA-deregulated T-lineage acute lymphoblastic leukemia: a Children's Oncology Group Study.

Leukemia·2016
Same author

Polychlorinated biphenyl congeners that increase the glucuronidation and biliary excretion of thyroxine are distinct from the congeners that enhance the serum disappearance of thyroxine.

Drug metabolism and disposition: the biological fate of chemicals·2011
Same author

Trimethyltin-induced alterations in behavior are linked to changes in PSA-NCAM expression.

Neurotoxicology·2006
Same author

Methylmercury alters Eph and ephrin expression during neuronal differentiation of P19 embryonal carcinoma cells.

Neurotoxicology·2005
Same author

The effects of steroidal estrogens in ACI rat mammary carcinogenesis: 17beta-estradiol, 2-hydroxyestradiol, 4-hydroxyestradiol, 16alpha-hydroxyestradiol, and 4-hydroxyestrone.

The Journal of endocrinology·2004

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • Pluripotent embryonal carcinoma (P19) cells are a valuable model for studying cellular differentiation.
  • Retinoic acid (RA) is a known inducer of neural differentiation in various cell types, including P19 cells.
  • Neurofilaments are critical structural components of neurons, and their subunit expression patterns are indicative of neuronal maturation.

Purpose of the Study:

  • To investigate the expression kinetics of the high molecular weight neurofilament subunit (NFH) during retinoic acid-induced neural differentiation of P19 cells.
  • To compare the temporal expression of NFH with low and medium molecular weight neurofilament subunits.
  • To determine if NFH expression in vitro mirrors patterns observed during in vivo neuronal development.

Main Methods:

Related Experiment Videos

  • Retinoic acid (RA) treatment of P19 embryonal carcinoma cells.
  • Western blotting to detect and quantify neurofilament subunit protein expression.
  • Immunofluorescence microscopy to visualize the localization and expression of neurofilament subunits within differentiating cells.

Main Results:

  • Retinoic acid (RA) treatment successfully induced neural differentiation in P19 cells.
  • High molecular weight neurofilament (NFH) subunit expression was detected, but significantly lagged behind the expression of low and medium molecular weight subunits.
  • The delayed onset of NFH expression in RA-treated P19 cells closely resembled temporal patterns observed during in vivo neuronal development.

Conclusions:

  • The temporal regulation of neurofilament subunit expression, particularly the delayed appearance of NFH, is conserved during in vitro RA-induced neural differentiation of P19 cells.
  • This study provides further evidence for P19 cell differentiation as a relevant model for investigating molecular mechanisms of neuronal development.
  • Understanding NFH expression dynamics contributes to the broader knowledge of neuronal maturation and cytoskeletal organization.