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Delayed expression of the NFH subunit in differentiating P19 cells
S J Pyle1, K G Roberts, K R Reuhl
1Biology Department, University of North Dakota, P.O. Box 9019, Grand Forks, ND 58202-9019, USA. sally_pyle@und.nodak.edu
Brain Research. Developmental Brain Research
|December 18, 2001
Abstract:
Pluripotent embryonal carcinoma (P19) cells differentiate into a neural phenotype in response to retinoic acid (RA). Expression of the low and medium molecular weight neurofilament subunits, but not the high molecular weight subunit (NFH), has been reported following RA treatment. In this study NFH expression was detected by Western blotting and immunofluorescence microscopy, but lagged behind the expression of the other subunits in a manner similar to that reported during in vivo neuronal development.