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Phase-fluorometry study on dielectric relaxation of acrylodan-labeled human serum albumin
A Buzády1, J Erostyák, B Somogyi
1Department of Experimental Physics, Institute of Physics, University of Pécs, H-7624 Pécs, Ifjúság u. 6., Hungary. buzady@fizika.ttk.pte.hu
Biophysical Chemistry
|December 18, 2001
Abstract:
Dielectric relaxation (DR) of acrylodan-labeled human serum albumin (HSA/AC) was studied by phase-fluorometry. A non-monoexponential behavior of both the total fluorescence--and the DR decays has been found. The protein environment of the fluorescent marker shows DR times ranging from the pico to nanosecond timescale. In fluorescence emission decays measured on the red side of the fluorescence spectrum a time constant (<10 ps) affected by a negative preexponential was found supporting the existence of DR of the excited states.