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Related Experiment Videos

Advances in cooled semen technology.

F Batellier1, M Vidament, J Fauquant

  • 1Equipe Reproduction, INRA, Unité SRA, 37380 Nouzilly, France.

Animal Reproduction Science
|December 18, 2001
PubMed
Summary
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A new extender, INRA96, improves stallion semen quality for artificial insemination. Storing semen with INRA96 at 15 degrees C offers an effective alternative to traditional milk-based extenders stored at 4 degrees C.

Area of Science:

  • Animal Science
  • Reproductive Biology
  • Veterinary Medicine

Background:

  • Artificial insemination (AI) in horses relies on chilled semen, but pregnancy rates are often low due to variable semen quality.
  • Traditional milk-based extenders are used for semen storage, but their efficacy can be limited.
  • Identifying improved semen extenders is crucial for enhancing equine reproductive success.

Purpose of the Study:

  • To evaluate a novel semen extender, INRA96, for cooling, storage, and transport of stallion semen.
  • To compare the efficacy of INRA96 with conventional milk-based extenders under various storage conditions.
  • To assess the impact of INRA96 on stallion sperm survival and subsequent pregnancy rates.

Main Methods:

  • Developed INRA96 extender using modified Hanks' salts and a purified milk fraction (native phosphocaseinate, NPPC).

Related Experiment Videos

  • Conducted four experiments comparing INRA96 with milk-based extenders (Kenney, INRA82, E-Z Mixin) under different storage temperatures (4°C and 15°C) and durations (24h to 3 days).
  • Assessed pregnancy rates per cycle following artificial insemination with treated semen.

    • Main Results:

    • INRA96 extender significantly improved pregnancy rates at 15°C compared to milk-based extenders at 4°C after 24h storage (57% vs. 40%).
    • Semen stored in INRA96 at 15°C for 3 days achieved a 48% pregnancy rate, comparable to immediate insemination (68%).
    • INRA96 demonstrated equal efficiency at 15°C and 4°C for 24h storage, suggesting flexibility in temperature management.

    Conclusions:

    • INRA96 extender provides a viable alternative to milk-based extenders for storing stallion semen, particularly for stallions with "poor cooler" characteristics.
    • Storage of stallion semen in INRA96 at 15°C is as effective as storage at 4°C for maintaining sperm motility and fertility.
    • The development of INRA96 offers a promising advancement for improving the efficiency and reliability of equine artificial insemination programs.