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Related Experiment Videos

Intralipid 10%: physicochemical characterization.

J Férézou1, A Gulik, N Domingo

  • 1Laboratoire de Physiologie de la Nutrition, Université Paris-Sud, Orsay, France.

Nutrition (Burbank, Los Angeles County, Calif.)
|December 18, 2001
PubMed
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Parenteral fat emulsions contain smaller liposomes than previously thought, with potential implications for patient reactions. This study characterized these liposomes and detected trace proteins.

Area of Science:

  • Lipid nanoparticle characterization
  • Parenteral nutrition
  • Biophysical chemistry

Background:

  • Parenteral fat emulsions are complex mixtures containing artificial chylomicrons and liposomes.
  • Liposomes, composed of phospholipid bilayers, encapsulate an aqueous phase and are isolatable via centrifugation.
  • The mesophase, an infranatant from centrifugation, is known to contain these liposomes.

Purpose of the Study:

  • To chemically characterize the mesophase of parenteral fat emulsions.
  • To visualize the particles within the mesophase using electron microscopy.
  • To investigate the composition and properties of liposomes in parenteral nutrition formulations.

Main Methods:

  • Cryofracture electron microscopy was used to examine native Intralipid, mesophase, and enriched liposome fractions.

Related Experiment Videos

  • Triacylglycerol (TAG) and protein content of the mesophase were quantified.
  • Proteins were partially characterized using Western-blotting with an antibody against the Anionic Polypeptide Factor (APF).

    • Main Results:

    • Artificial chylomicrons (260 nm) and smaller-than-expected liposomes (43 nm) were observed in 10% Intralipid.
    • Purified liposomes contained 8% TAG, exceeding the typical bilayer solubility limit.
    • Minute amounts of proteins, including those interacting with phospholipids, were detected.

    Conclusions:

    • Liposomes in 10% Intralipid have a mean diameter of 43 nm, smaller than previously assumed.
    • Increased TAG solubilization in phospholipid bilayers may be influenced by manufacturing or high TAG/PL ratios.
    • Trace hydrophobic proteins in parenteral emulsions could be linked to allergic reactions in patients.