Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A simple method for detecting antibodies to rubella.

A Voller, D E Bidwell

    British Journal of Experimental Pathology
    |August 1, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Evaluation of a malaria antibody ELISA and its value in reducing potential wastage of red cell donations from blood donors exposed to malaria, with a note on a case of transfusion-transmitted malaria.

    Vox sanguinis·1997
    Same author

    Evaluation of a malaria antigen ELISA.

    Transactions of the Royal Society of Tropical Medicine and Hygiene·1994
    Same author

    Immunoassays for tropical parasitic infections.

    Transactions of the Royal Society of Tropical Medicine and Hygiene·1993
    Same author

    Diagnosis of human toxocariasis by antigen capture enzyme linked immunosorbent assay.

    Journal of clinical pathology·1993
    Same author

    Progress toward a simplified polymerase chain reaction and its application to diagnosis of tuberculosis.

    Journal of clinical microbiology·1993
    Same author

    False-positive serology and HIV infection.

    Lancet (London, England)·1993

    A new microplate enzyme-linked immunosorbent assay (ELISA) for Rubella antibody detection is presented. This Micro-ELISA showed high agreement with the established haemagglutination inhibition test in human serum samples.

    Area of Science:

    • Immunology
    • Virology
    • Diagnostic Assays

    Background:

    • Rubella virus infections pose risks, necessitating accurate antibody detection.
    • Traditional diagnostic methods like haemagglutination inhibition (HI) are established but can be complex.
    • Development of simpler, efficient diagnostic assays is crucial for widespread screening.

    Purpose of the Study:

    • To describe a novel microplate enzyme-linked immunosorbent assay (Micro-ELISA) for quantifying Rubella-specific antibodies.
    • To evaluate the performance of the Micro-ELISA by comparing it with the haemagglutination inhibition (HI) test.
    • To assess the diagnostic accuracy and concordance of the new assay.

    Main Methods:

    • A simple microplate enzyme-linked immunosorbent assay (Micro-ELISA) was developed for Rubella antibody detection.

    Related Experiment Videos

  • The Micro-ELISA was validated against the haemagglutination inhibition (HI) test.
  • A study involving 188 human serum samples was conducted for comparative analysis.

    • Main Results:

    • The newly developed Micro-ELISA provides a straightforward method for Rubella antibody assessment.
    • Comparison with the haemagglutination inhibition test revealed a low total discrepancy rate of only 3.7%.
    • The results indicate high concordance between the Micro-ELISA and the HI test.

    Conclusions:

    • The Micro-ELISA is a simple and effective method for detecting Rubella antibodies.
    • This assay demonstrates excellent agreement with the haemagglutination inhibition test.
    • The Micro-ELISA offers a reliable alternative for Rubella serological testing.