Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional interaction between p/CAF and human papillomavirus E2 protein.

Daeyoup Lee1, Sun Gwan Hwang, Jiyun Kim

  • 1Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.

The Journal of Biological Chemistry
|December 18, 2001
PubMed
Summary

p300/CREB-binding protein-associated factor (p/CAF) acts as a co-activator for human papillomavirus E2 protein. Its histone acetylase activity is crucial for activating E2-dependent transcription, suggesting a key role in viral gene expression.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Blood-based gene signatures associated with therapeutic response to anti-TNF therapy in rheumatoid arthritis: a combined meta-analytical and machine learning approach.

EULAR rheumatology open·2026
Same author

Evaluation of Dithio Compounds Modified Porous Silicon for Label-Free Detection and Distinction of Biothiols.

ACS applied materials & interfaces·2026
Same author

A Unique Case of Methemoglobinemia Presenting With ST Elevation Myocardial Infarction, Shock, and Left Ventricular Dysfunction.

The Canadian journal of cardiology·2026
Same author

Association between high-risk drinking and cardiovascular health based on Life's Essential 8: analysis using 2016-2021 Korean National Health and Nutrition Examination Survey data.

BMJ open·2026
Same author

Bioinspired Ion Doping for Threshold Control in Green Chitosan-Based Flexible Transistor Neuromorphic Devices.

ACS applied materials & interfaces·2026
Same author

QAL333's antitumor activity and predictive modeling: integrated transcriptomic-bioinformatic analysis reveals selective cytotoxicity and sensitivity determinants.

Scientific reports·2026

Area of Science:

  • Molecular Biology
  • Virology
  • Epigenetics

Background:

  • p300/CREB-binding protein-associated factor (p/CAF) is a known transcriptional co-activator.
  • p/CAF interacts with p300/CBP and acidic transcription factors, mediating transcription via histone and cellular factor acetylation.
  • Previous research established that CBP binds to human papillomavirus (HPV) E2 protein and activates E2-dependent transcription.

Purpose of the Study:

  • To investigate the interaction between p/CAF and HPV E2 protein.
  • To determine if p/CAF functions as a co-activator for HPV E2.
  • To elucidate the role of p/CAF's histone acetylase activity in E2-dependent transcription.

Main Methods:

  • In vivo and in vitro binding assays to assess p/CAF and HPV E2 interaction.

Related Experiment Videos

  • Transcriptional assays to measure E2-dependent gene activation.
  • Assays to evaluate the necessity of p/CAF's histone acetylase activity for transcriptional activation.

    • Main Results:

    • p/CAF was shown to bind to the HPV E2 protein both in vivo and in vitro.
    • p/CAF was demonstrated to activate E2-dependent transcription.
    • CBP and p/CAF synergistically enhanced E2-dependent transcription.
    • The histone acetylase activity of p/CAF was found to be essential for efficient activation of E2 transcriptional activity.

    Conclusions:

    • p/CAF physically interacts with the human papillomavirus E2 protein.
    • p/CAF functions as a transcriptional co-activator for the HPV E2 protein.
    • p/CAF's enzymatic activity, specifically histone acetylation, is critical for its co-activator function with HPV E2.