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Related Experiment Videos

Murine keratocytes function as antigen-presenting cells.

S K Seo1, B M Gebhardt, H Y Lim

  • 1The Immunomodulation Research Center and Department of Biological Sciences, University of Ulsan, Ulsan, Korea.

European Journal of Immunology
|December 18, 2001
PubMed
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Corneal keratocytes act as antigen-presenting cells, providing crucial costimulatory signals to T cells. This function is vital for immune responses in the cornea, potentially influencing conditions like herpetic stromal keratitis.

Area of Science:

  • Immunology
  • Ophthalmology
  • Cell Biology

Background:

  • Keratocytes constitutively express MHC class I and costimulatory molecules like B7-1 and ICAM-1.
  • IFN-gamma stimulation induces MHC class II expression on keratocytes.
  • These molecules suggest keratocytes can present antigens and provide costimulatory signals to T cells.

Purpose of the Study:

  • To investigate the antigen-presenting and costimulatory capabilities of mouse corneal keratocytes.
  • To determine if keratocytes can activate T cells in an antigen-specific manner.
  • To explore the role of keratocytes in immune-mediated corneal inflammation.

Main Methods:

  • Incubation of mouse T cells (CD4+ and CD8+) with irradiated keratocytes.
  • Use of anti-CD3 mAb and anti-B7-1 mAb to assess T cell proliferation and costimulation.

Related Experiment Videos

  • Incubation of T cells from herpes-infected mice with HSV-1-infected keratocytes to evaluate antigen-specific activation.
  • Measurement of T cell proliferation and Th1 cytokine production (IL-2, IFN-gamma).

    • Main Results:

    • Keratocytes expressing B7-1 provided a costimulatory signal to both CD4+ and CD8+ T cells, enhancing their proliferation.
    • This costimulatory effect was inhibited by anti-B7-1 mAb.
    • HSV-1-infected keratocytes activated T cells from herpes-infected mice, leading to proliferation and Th1 cytokine production.
    • Keratocytes presented viral antigens, demonstrating antigen-specific T cell activation.

    Conclusions:

    • Mouse corneal keratocytes function as potent antigen-presenting cells.
    • Keratocytes provide both antigen-specific and costimulatory signals essential for T cell activation.
    • These findings highlight the role of keratocytes in initiating and modulating immune responses within the corneal stroma.
    • Keratocytes may contribute to immune-mediated stromal keratitis, including herpetic stromal keratitis.