Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The natural substrate for nitric oxide synthase activity.

J Alaghband-Zadeh1, S Mehdizadeh, N S Khan

  • 1Department of Clinical Chemistry, Imperial College School of Medicine, Charing Cross Campus, London, UK.

Cell Biochemistry and Function
|December 18, 2001
PubMed
Summary

Carnosine, not arginine, is likely the natural substrate for nitric oxide synthase (NOS) activity. Carnosine demonstrates a stronger NOS reaction in tissue sections, suggesting its primary role in NO generation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Is COVID-19 finally just a bad flu? Follow-up study comparing disease severity among COVID-19 and seasonal influenza hospital in-patients across pandemic waves in Ireland.

Public health·2024
Same author

Next Stop a Longer Life.

Irish medical journal·2020
Same author

A qualitative study of the perceived impact of a community mobilisation intervention to reduce alcohol consumption among amateur sportsmen.

Irish journal of psychological medicine·2018
Same author

Is Increasing Life Expectancy Leading To More Complexity?

Irish medical journal·2018
Same author

The Epidemiology of Emergency In-Patient Hospitalisations Among Those with 'No Fixed Abode' (Homeless) 2005-2014: What Lessons Can Be Learnt.

Irish medical journal·2017
Same author

Temporal changes in bile acid levels and 12α-hydroxylation after Roux-en-Y gastric bypass surgery in type 2 diabetes.

International journal of obesity (2005)·2016

Area of Science:

  • Biochemistry
  • Physiology

Background:

  • Nitric oxide synthase (NOS) activity is crucial for various physiological processes.
  • Arginine has been traditionally considered the primary substrate for NOS, but evidence remains limited.

Purpose of the Study:

  • To investigate the natural substrate responsible for nitric oxide synthase (NOS) activity.
  • To compare the efficacy of carnosine versus arginine as NOS substrates.

Main Methods:

  • Histochemical analysis of tissue sections.
  • Assessment of NOS reaction intensity with carnosine and arginine.

Main Results:

  • Carnosine is widely distributed in various tissues.
  • Carnosine yielded a significantly stronger NOS reaction compared to arginine in tissue sections.

Related Experiment Videos

Conclusions:

  • Carnosine is likely the true natural substrate for NOS activity.
  • These findings challenge the established role of arginine in NO generation and highlight carnosine's importance.