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Updated: Jul 18, 2026
An Orthotopic Murine Model of Human Prostate Cancer Metastasis
Published on: September 18, 2013
Stable lower PAR expression decreased DU145 prostate cancer cell growth in SCID mice
1Department of Medicine, Mount Sinai Medical Center, New York, New York 10029, USA. mickey.platica@mssm.edu
Background:
PAR is a novel gene ubiquitously expressed in normal and malignant tissues with a trend towards higher expression in tumor cells. PAR biological function is unknown. Here we report the effect of lowering PAR expression on in vitro and in vivo proliferation of DU145 cells.
Methods:
Decreased PAR expression was achieved by stable transfection of DU145 cells with antisense PAR cDNA cloned in pCMV-Script expression vector. The proliferative potential of DU145 transfectants was studied by cell counts, colony formation in soft agar, flow cytometry, and growth in severe combined immunodeficient (SCID) mice.
Results:
DU145 transfectants exhibited a decreased cell proliferation in tissue culture and a low efficiency of colony formation in soft agar. Flow cytometry revealed an arrest of these cells in G2-M phase of mitotic cycle. A dramatic decrease of tumor growth was observed when DU145 transfectant cells were inoculated in SCID mice, compared with controls. Histological examination of these tumors showed a marked decrease in cell density and in number of mitoses while control tumors showed a high cell density and numerous mitoses.
Conclusions:
The data presented here provide the first evidence for PAR gene cellular function and its possible implication in malignant transformation.
Insights
Lowering PAR gene expression significantly inhibits DU145 cell proliferation in vitro and reduces tumor growth in vivo. This study reveals PAR
Area of Science:
- Molecular Biology
- Cancer Research
- Cell Biology
Background:
- The PAR gene is widely expressed in normal and tumor tissues, often upregulated in cancer.
- The specific biological role of PAR remains largely uncharacterized.
Purpose of the Study:
- To investigate the cellular function of the PAR gene.
- To determine the effect of reduced PAR expression on DU145 cell proliferation.
Main Methods:
- DU145 cells were stably transfected with antisense PAR cDNA.
- Proliferation was assessed via cell counts, soft agar colony formation, and flow cytometry.
- In vivo tumor growth was evaluated in SCID mice.
Main Results:
- Transfected DU145 cells showed reduced proliferation and soft agar colony formation.
- Flow cytometry indicated a G2-M phase cell cycle arrest.
- Tumor growth in SCID mice was significantly inhibited, with decreased cell density and mitoses.
Conclusions:
- This study provides the first evidence for the cellular function of the PAR gene.
- PAR gene expression may play a role in malignant transformation.
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