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Study of the mode of cyclophosphamide action on the rabbit arthritis according to Glynn's model
A knee joint arthritis is induced in rabbits previously immunized by an intradermal injection of emulsified egg albumin (E.A.) a complete adjuvant, then by an intra-articular injection of E.A. alone. Histologically, the synovium shows at first an Arthus phenomenon and later an immune cellular reaction. Several immunological parameters are studied in these rabbits. Cyclophosphamide (CY), injected intravenously, in a high dose, during a short period from the day of the first immunization, inhibits in the same time the arthritis index, E.A. skin test, humoral and synovial anti-E.A. antibodies and lymphocyte response to P.H.A. Cy, injected I.V., in a low dose, for a long period from the day of the intra-articular immunization, decreases only the arthritis index, but does not modify the various immune reactions to E.A. These results suggest that, according to the timing and the dose, CY inhibits synovial inflammation either by a strict anti-inflammatory action, or by an immunosuppressive effect on the initial phase of the immune reaction inducing subsequently a synovial inflammation.
A knee joint arthritis is induced in rabbits previously immunized by an intradermal injection of emulsified egg albumin (E.A.) a complete adjuvant, then by an intra-articular injection of E.A. alone. Histologically, the synovium shows at first an Arthus phenomenon and later an immune cellular reaction. Several immunological parameters are studied in these rabbits. Cyclophosphamide (CY), injected intravenously, in a high dose, during a short period from the day of the first immunization, inhibits in the same time the arthritis index, E.A. skin test, humoral and synovial anti-E.A. antibodies and lymphocyte response to P.H.A. Cy, injected I.V., in a low dose, for a long period from the day of the intra-articular immunization, decreases only the arthritis index, but does not modify the various immune reactions to E.A. These results suggest that, according to the timing and the dose, CY inhibits synovial inflammation either by a strict anti-inflammatory action, or by an immunosuppressive effect on the initial phase of the immune reaction inducing subsequently a synovial inflammation.