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A human RNA polymerase II subunit is encoded by a recently generated multigene family
S Grandemange1, S Schaller, S Yamano
1Institut de Génétique et de Biologie Moléculaire et Cellulaire (CNRS / INSERM / ULP) BP 163, F-67404 ILLKIRCH Cedex, France. sylviegrandemange@hotmail.com
BMC Molecular Biology
|December 19, 2001
Summary
Human RNA polymerase II subunit 11 (hRPB11) is encoded by a multigene family, unlike yeast RPB. The hRPB11b gene likely arose from primate evolution involving mismatch repair (MMR) gene elements.
Area of Science:
- Molecular Biology
- Genetics
- Evolutionary Biology
Background:
- Yeast RNA polymerase II (RPB) subunit genes are unique.
- Human RPB subunits characterized thus far are also unique.
Purpose of the Study:
- Investigate the gene structure of human RNA polymerase II subunit 11 (hRPB11).
- Determine the functional differences between hRPB11 gene family members.
Main Methods:
- Gene mapping and sequencing
- Differential splicing analysis
- Interspecies complementation assays
Main Results:
- hRPB11 is encoded by a multigene family on human chromosome 7.
- Two family members, hRPB11a and hRPB11b, were identified.
- hRPB11b exhibits differential splicing and homology to mismatch repair (MMR) genes.
- Only hRPB11balpha is functional in yeast.
- The murine RPB11 homolog is unique and identical to hRPB11a.
Conclusions:
- The hRPB11b gene likely resulted from recent genomic recombination in primate evolution.
- These recombination events involved sequence elements related to the MMR apparatus.
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