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Updated: Jul 25, 2026
Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis
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Generation of a selective 5-HT3B subunit-recognising polyclonal antibody; identification of immunoreactive cells in
1Department of Pharmacology, The Medical School, University of Birmingham, Edgbaston, B15 2TT, Birmingham, UK.
Abstract:
The present study generated a polyclonal antibody (AP86/3) that recognises a peptide sequence of the h5-HT(3B) receptor subunit. Western blot analysis of homogenates prepared from cell lines expressing either homomeric (h5-HT(3A)) or heteromeric (h5-HT(3A/3B)) receptors, as well as immunocytochemical studies with the same cell lines, indicated that AP86/3 recognised, selectively, the 5-HT(3B) subunit. Immunohistochemical labelling was also apparent in cells in the rat hippocampus that displayed the distribution and morphology of interneurones.
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