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Related Experiment Videos

Patterning the optic neuroepithelium by FGF signaling and Ras activation.

S Zhao1, F C Hung, J S Colvin

  • 1Department of Molecular and Cellular Biology, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.

Development (Cambridge, England)
|December 19, 2001
PubMed
Summary
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Fibroblast Growth Factor 9 (FGF9) is crucial for neural patterning in the vertebrate eye. FGF9 directs the differentiation of neural retina and prevents retinal pigment epithelium from becoming neural tissue.

Area of Science:

  • Developmental Biology
  • Neuroscience
  • Molecular Biology

Background:

  • Vertebrate embryogenesis involves neuroectoderm differentiating into both neural and non-neural tissues.
  • Mechanisms patterning neural and non-neural tissues within the neuroectoderm are not fully understood.
  • The optic vesicle is a key structure in eye development, differentiating into neural retina and retinal pigment epithelium.

Purpose of the Study:

  • To investigate the role of FGF9 in neural patterning within the optic neuroepithelium.
  • To elucidate the molecular mechanisms underlying the differentiation of neural and non-neural tissues in the developing eye.
  • To explore the involvement of Ras-mediated signaling in optic vesicle neural differentiation.

Main Methods:

  • Analysis of FGF9 expression patterns in the developing optic vesicle.

Related Experiment Videos

  • Generating transgenic mice with ectopic FGF9 expression.
  • Generating transgenic mice with ectopic expression of constitutively active Ras.
  • Studying mouse embryos lacking FGF9.

    • Main Results:

    • FGF9 is expressed in the distal optic vesicle, destined for neural retina formation.
    • Ectopic FGF9 expression induced neural differentiation in the presumptive retinal pigment epithelium, creating a duplicate neural retina.
    • Constitutively active Ras also converted retinal pigment epithelium to neural retina.
    • FGF9 deficiency led to retinal pigment epithelium invasion into the presumptive neural retina.

    Conclusions:

    • FGF9 plays a critical role in neural patterning and defining the boundary between neural retina and retinal pigment epithelium.
    • Ras-mediated signaling is implicated in neural differentiation within the optic vesicle.
    • Retinal neuronal differentiation appears to be autonomously regulated, even without retinal pigment epithelium.