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Diagnosis of Oropouche virus infection by RT-nested-PCR
Marcos Lázaro Moreli1, Victor Hugo Aquino, Ana Cecília R Cruz
1Virus Research Unit, School of Medicine of Ribeirão Preto, University of São Paulo, Campus da USP, Ribeirão Preto, SP, Brazil.
Abstract:
Using the RT-PCR with primers that anneal to the 5' and the 3' extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients. These results show that this RT-nested-PCR is a useful tool for rapid diagnosis of Oropouche fever infections.
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