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Related Experiment Videos

Emulsion-derived foams (PolyHIPEs) containing poly(epsilon-caprolactone) as matrixes for tissue engineering.

W Busby1, N R Cameron, C A Jahoda

  • 1Department of Chemistry, University of Durham, South Road, Durham DH1 3LE, UK.

Biomacromolecules
|December 26, 2001
PubMed
Summary

Poly(epsilon-caprolactone) macromonomers create PolyHIPE foams via free radical polymerization. Foam properties like morphology and swelling depend on diluents and poly(epsilon-caprolactone) content, with potential for cell growth.

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Area of Science:

  • Polymer Chemistry
  • Materials Science
  • Biomaterials

Background:

  • High internal phase emulsions (HIPEs) are versatile templates for creating porous materials.
  • Poly(epsilon-caprolactone) (PCL) is a biodegradable polyester with tunable properties.
  • Macromonomer polymerization offers control over polymer network architecture.

Purpose of the Study:

  • To synthesize and characterize poly(epsilon-caprolactone) (PCL)-containing PolyHIPE foams.
  • To investigate the influence of synthesis parameters on foam morphology and properties.
  • To evaluate the swelling behavior and potential biocompatibility of the developed foams.

Main Methods:

  • Synthesis of PCL macromonomers from PCL diols.
  • Free radical homo- or copolymerization of macromonomers in HIPEs.

Related Experiment Videos

  • Characterization of foam morphology using scanning electron microscopy.
  • Assessment of foam swelling via solvent imbibition.
  • Evaluation of human fibroblast cell growth on foam samples.

    • Main Results:

    • Low-density PolyHIPE foams with 5-100 micrometer cell diameters were successfully prepared.
    • Foam morphology was significantly influenced by the choice of diluent (styrene, MMA, toluene) and PCL content.
    • Increasing PCL content enhanced continuous phase viscosity, potentially hindering emulsion formation.
    • Foam swelling increased with solvent hydrophobicity and decreased with higher PCL content due to cross-linking.
    • Higher molar mass PCL macromonomers resulted in less tightly cross-linked networks and increased swelling.
    • One foam type supported human fibroblast growth for 2.5 days.

    Conclusions:

    • PolyHIPE foams incorporating PCL can be controllably synthesized using macromonomer polymerization.
    • Foam structure and solvent interaction are tunable via PCL content, macromonomer molar mass, and diluent selection.
    • The developed PCL-based PolyHIPE foams show promise as potential scaffolds for tissue engineering applications.