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NO mediates ginsenoside Rg1-induced long-term potentiation in anesthetized rats
1Department of Pharmacology, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, China.
Aim:
To investigate the effect of ginsenoside Rg1 on synaptic transmission in anesthetized rats and the effect of NOS inhibitor, 7-nitroindazole (7-NI), on long-term potentiation (LTP) induced by Rg1.
Methods:
Extracellular recording technique was used to record the population spike (PS) in the dentate gyrus (DG) of anesthetized rats. Drug or vehicle injections were delivered via a cannula in the lateral cerebral ventricle.
Results:
Rg1 (10 and 100 nmol/L) enhanced the basic synaptic transmission and the magnitude of LTP induced by high frequency stimulation (HFS). Selective nNOS inhibitor 7-NI (5 nmol) icv could inhibit the induction of perforant path-dentate gyrus LTP elicited by Rg1 (P<0.05), and L-arginine 250 g/L ip prevented the action of 7-nitroindazole (P<0.05).
Conclusion:
Rg1-accelerated synaptic transmission and nitric oxide produced by nNOS played a role in the induction of PP-DG LTP in anesthetized rats.