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Cloning a novel mouse Gabarapl2 cDNA and its characterization
1School of Life Science, Suzhou University, Suzhou 215006, China. zhengchen_99@yahoo.com
Acta Pharmacologica Sinica
|December 26, 2001
Summary
Researchers cloned the novel mouse GABAA-receptor-associated protein like 2 (Gabarapl2) gene. This study identified Gabarapl2 expression patterns in various mouse tissues, contributing to understanding its function.
Area of Science:
- Molecular Biology
- Genetics
Background:
- GABAA-receptor-associated proteins play crucial roles in neuronal function.
- Understanding the diversity of these proteins is essential for neuroscience research.
Purpose of the Study:
- To clone and characterize a novel mouse GABAA-receptor-associated protein like 2 (Gabarapl2) gene.
- To investigate the expression profile of the cloned mouse Gabarapl2 gene in various tissues.
Main Methods:
- Utilized human GABARAPL2 cDNA as a probe to identify homologous sequences in the mouse EST database.
- Assembled overlapping ESTs into a contig using the GCG ASSEMBLY program.
- Confirmed gene existence and analyzed expression patterns via Northern blotting across 11 mouse tissues.
Main Results:
- Successfully cloned and identified the novel mouse Gabarapl2 gene (GenBank Accession No AF190644).
- The deduced protein is identical to human GABARAPL2, with predicted phosphorylation sites.
- Northern hybridization revealed a 1.35 kb transcript expressed in brain, thymus, lung, heart, kidney, and liver, with lower levels in other tissues.
Conclusions:
- A novel mouse Gabarapl2 gene has been successfully cloned and identified.
- The study provides foundational data on Gabarapl2 gene expression in mice.