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Related Experiment Videos

Cysteine oxidase in brain.

C H Misra, J W Olney

    Brain Research
    |October 24, 1975
    PubMed
    Summary
    This summary is machine-generated.

    Cysteine oxidase activity in rat brains was successfully measured for the first time. This enzyme

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    Area of Science:

    • Neuroscience
    • Biochemistry

    Background:

    • Cysteine oxidase catalyzes cysteine oxidation to neuroactive compounds.
    • Previous studies failed to successfully measure brain cysteine oxidase activity.

    Purpose of the Study:

    • Establish optimal conditions for measuring rat brain cysteine oxidase.
    • Determine regional and subcellular distribution.
    • Analyze developmental changes in activity.

    Main Methods:

    • Utilized NAD+ as a cofactor for enzyme activity measurement.
    • Assessed activity in whole brain homogenate and subcellular fractions.
    • Compared activity across different brain regions and developmental stages.

    Main Results:

    • Substantial cysteine oxidase activity was measurable in rat brain homogenate.

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  • 70% of the activity was localized to the microsomal fraction.
  • Activity varied regionally, with highest levels in pons-medulla and lowest in cerebral cortex.
  • Adult rat brain showed approximately 5-fold higher activity than at birth.
  • Activity increased significantly after the first 6 days of life, reaching adult levels by 25-50 days.

    • Conclusions:

    • Optimal conditions for measuring rat brain cysteine oxidase activity have been established.
    • Cysteine oxidase is present in various brain regions and subcellular locations.
    • Enzyme activity undergoes significant developmental changes, increasing substantially after birth.