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Updated: Aug 18, 2026

Isolation of Physiologically Active Thylakoids and Their Use in Energy-Dependent Protein Transport Assays
Published on: September 28, 2018
Post-translational protein translocation into thylakoids by the Sec and DeltapH-dependent pathways
1Horticultural Sciences and Plant Molecular and Cellular Biology, University of Florida, 1137 Fifield Hall, Gainesville, FL 32611, USA.
Abstract:
Two distinct protein translocation pathways that employ hydrophobic signal peptides function in the plant thylakoid membrane. These two systems are precursor specific and distinguished by their energy and component requirements. Recent studies have shown that one pathway is homologous to the bacterial general export system called Sec. The other one, called the DeltapH-dependent pathway, was originally considered to be unique to plant thylakoids. However, it is now known that homologous transport systems are widely present in prokaryotes and even present in archaea. Here we review these protein transport pathways and discuss their capabilities and mechanisms of operation.
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